Description
EnzymeQA(iphone) is an App containing
about 160 revision questions and answers on enzymology. It is
restricted to considering single substrate catalysis. It is hoped that
it will help University undergraduate and postgraduate students revise
enzymology at home or on the go. It is not an exhaustive treatment of
enzymology. However, it is hoped that it will enable you to test your
knowledge of key facts in enzymology.
For those of you planning to determine kcat, kcat/Km and Km, EnzymeQA should help you check that you have the appropriate experimental conditions for determining catalytic constants. Likewise it should help planning to determine Ki values for competitive inhibition.
In iPhone version 2 the number of questions has been increased from about 80 to about 130. It is now a 5 page App as listed below.
In iPhone version 2.1 the number of questions has been increased to about 160. In this version the app has been updated and 26 new questions and answers have been added. There are new questions on; spectrophotometric and fluorescence assays, FRET assays, the advantages and disadvantages of plate reader assays, stopped flow and pH.
In iPhone version 2.2
Minor bug fixes ensuring compatibility with multiple devices.
In iPhone version 2.3
Some new pH questions have been added and some answers have been extended. The screenshots and App have been updated.
Topics include
Window 1
Michaelis Menten equation.
Initial rate measurements.
Setting the appropriate concentrations of enzyme and substrate for determining catalytic parameters.
Defining Vm, kcat, Km, kcat/Km, Ki and IC50 values.
Units of catalytic constants.
Order in enzyme and substrate at various substrate concentrations.
Methods for determining catalytic constants.
Experimental conditions for measuring catalytic constants.
Activities and Specific Activities.
Determining kcat/Km by complete turnover of substrates.
Window 2
Competitive inhibition.
Determining Ki values for competitive inhibition.
Relationship between Ki and IC50 values.
Brief mention of noncompetitive, mixed and uncompetitive inhibition.
Window 3
pH and pH values
Analysis of pH data
log plots
pH dependence of catalytic constants.
Window 4
Elisa
Enzyme cycling
Spectrophotometric versus fluorescence assays
FRET assays
Plate reader enzyme assays
Stopped flow
Window 5
Transition state analogues
Catalytic antibodies
Ribozymes
For those of you planning to determine kcat, kcat/Km and Km, EnzymeQA should help you check that you have the appropriate experimental conditions for determining catalytic constants. Likewise it should help planning to determine Ki values for competitive inhibition.
In iPhone version 2 the number of questions has been increased from about 80 to about 130. It is now a 5 page App as listed below.
In iPhone version 2.1 the number of questions has been increased to about 160. In this version the app has been updated and 26 new questions and answers have been added. There are new questions on; spectrophotometric and fluorescence assays, FRET assays, the advantages and disadvantages of plate reader assays, stopped flow and pH.
In iPhone version 2.2
Minor bug fixes ensuring compatibility with multiple devices.
In iPhone version 2.3
Some new pH questions have been added and some answers have been extended. The screenshots and App have been updated.
Topics include
Window 1
Michaelis Menten equation.
Initial rate measurements.
Setting the appropriate concentrations of enzyme and substrate for determining catalytic parameters.
Defining Vm, kcat, Km, kcat/Km, Ki and IC50 values.
Units of catalytic constants.
Order in enzyme and substrate at various substrate concentrations.
Methods for determining catalytic constants.
Experimental conditions for measuring catalytic constants.
Activities and Specific Activities.
Determining kcat/Km by complete turnover of substrates.
Window 2
Competitive inhibition.
Determining Ki values for competitive inhibition.
Relationship between Ki and IC50 values.
Brief mention of noncompetitive, mixed and uncompetitive inhibition.
Window 3
pH and pH values
Analysis of pH data
log plots
pH dependence of catalytic constants.
Window 4
Elisa
Enzyme cycling
Spectrophotometric versus fluorescence assays
FRET assays
Plate reader enzyme assays
Stopped flow
Window 5
Transition state analogues
Catalytic antibodies
Ribozymes
Instructions for use
When the application first opens press the "New Question" button
When the application first opens press the "New Question" button
A question will appear above the the "New Question" button.
Press the "Show Answer" button to get the answer to the current question.
When you press the "New Question" button again a new question will appear above the the "New Question" button.
"Answer" will appear above the "Show Answer button
Press the "Show Answer" button to get the answer to the current question.
If you quit the application, then when you re-open the application it should open at the last question you viewed.
There are about 160 questions so far.
Please note you cannot go backwards but all questions will be repeated as each cycle of questions is completed.
If you have any questions please contact
jpgmprograms@gmail.com